PoplarV1, Main, Exploration, bibRecord, 000237

Overexpression of PtDefensin enhances resistance to Septotis populiperda in transgenic poplar.

Identifieur interne : 000237 ( Main/Exploration ); précédent : 000236; suivant : 000238

Overexpression of PtDefensin enhances resistance to Septotis populiperda in transgenic poplar.

Auteurs : Hui Wei [République populaire de Chine] ; Ali Movahedi [République populaire de Chine] ; Chen Xu [République populaire de Chine] ; Weibo Sun [République populaire de Chine] ; Xiaoli Wang [République populaire de Chine] ; Dawei Li [République populaire de Chine] ; Qiang Zhuge [République populaire de Chine]

Source :

Plant science : an international journal of experimental plant biology [ 1873-2259 ] ; 2020.

RBID : pubmed:32005384

Descripteurs français

KwdFr :
- Ascomycota (physiologie), Défensines (génétique), Défensines (métabolisme), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Populus (génétique), Populus (microbiologie), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Résistance à la maladie (MeSH), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (microbiologie).
MESH :
- génétique : Défensines, Maladies des plantes, Populus, Protéines végétales, Végétaux génétiquement modifiés.
- microbiologie : Maladies des plantes, Populus, Végétaux génétiquement modifiés.
- métabolisme : Défensines, Protéines végétales.
- physiologie : Ascomycota.
- Régulation de l'expression des gènes végétaux, Résistance à la maladie.

English descriptors

KwdEn :
- Ascomycota (physiology), Defensins (genetics), Defensins (metabolism), Disease Resistance (MeSH), Gene Expression Regulation, Plant (MeSH), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (microbiology), Populus (genetics), Populus (microbiology).
MESH :
- chemical , genetics : Defensins, Plant Proteins.
- chemical , metabolism : Defensins, Plant Proteins.
- genetics : Plant Diseases, Plants, Genetically Modified, Populus.
- microbiology : Plant Diseases, Plants, Genetically Modified, Populus.
- physiology : Ascomycota.
- Disease Resistance, Gene Expression Regulation, Plant.

Abstract

Plant defensins have been implicated in the plant defense system, but their role in poplar immunity is still unclear. In the present study, we present evidence that PtDefensin, a putative plant defensin, participates in the defense of poplar plants against Septotis populiperda infection. After the construction of recombinant plasmid PET-32a-PtDefensin, PtDefensin protein was expressed in Escherichia coli strain BL21 (DE3) and purified through Ni-IDA resin affinity chromatography. The Trx-PtDefensin fusion protein displayed no cytotoxic activity against RAW264.7 cells but had cytotoxic activity against E. coli K12D31 cells. Analyses of PtDefensin transcript abundance showed that the expression levels of PtDefensin responded to abiotic and biotic stresses. Overexpression of PtDefensin in 'Nanlin 895' poplars (Populus × euramericana cv 'Nanlin895') increased resistance to Septotis populiperda, coupled with upregulation of MYC2 (basic helix-loop-helix (bHLH) transcription factor) related to jasmonic acid (JA) signal transduction pathways and downregulation of Jasmonate-zim domain (JAZ), an inhibitor in the JA signal transduction pathway. We speculate that systemic acquired resistance (SAR) was activated in non-transgenic poplars after S. populiperda incubation, and that induced systemic resistance (ISR) was activated more obviously in transgenic poplars after S. populiperda incubation. Hence, overexpression of PtDefensin may improve the resistance of poplar plants to pathogens.

DOI: 10.1016/j.plantsci.2019.110379
PubMed: 32005384

Affiliations:

République populaire de Chine

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Le document en format XML

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<term>Defensins (genetics)</term>
<term>Defensins (metabolism)</term>
<term>Disease Resistance (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Plant Diseases (genetics)</term>
<term>Plant Diseases (microbiology)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (microbiology)</term>
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<term>Populus (microbiology)</term>
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<term>Défensines (génétique)</term>
<term>Défensines (métabolisme)</term>
<term>Maladies des plantes (génétique)</term>
<term>Maladies des plantes (microbiologie)</term>
<term>Populus (génétique)</term>
<term>Populus (microbiologie)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Résistance à la maladie (MeSH)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Végétaux génétiquement modifiés (microbiologie)</term>
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<term>Plant Proteins</term>
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<term>Maladies des plantes</term>
<term>Populus</term>
<term>Protéines végétales</term>
<term>Végétaux génétiquement modifiés</term>
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<term>Végétaux génétiquement modifiés</term>
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<term>Populus</term>
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<front><div type="abstract" xml:lang="en">Plant defensins have been implicated in the plant defense system, but their role in poplar immunity is still unclear. In the present study, we present evidence that PtDefensin, a putative plant defensin, participates in the defense of poplar plants against Septotis populiperda infection. After the construction of recombinant plasmid PET-32a-PtDefensin, PtDefensin protein was expressed in Escherichia coli strain BL21 (DE3) and purified through Ni-IDA resin affinity chromatography. The Trx-PtDefensin fusion protein displayed no cytotoxic activity against RAW264.7 cells but had cytotoxic activity against E. coli K12D31 cells. Analyses of PtDefensin transcript abundance showed that the expression levels of PtDefensin responded to abiotic and biotic stresses. Overexpression of PtDefensin in 'Nanlin 895' poplars (Populus × euramericana cv 'Nanlin895') increased resistance to Septotis populiperda, coupled with upregulation of MYC2 (basic helix-loop-helix (bHLH) transcription factor) related to jasmonic acid (JA) signal transduction pathways and downregulation of Jasmonate-zim domain (JAZ), an inhibitor in the JA signal transduction pathway. We speculate that systemic acquired resistance (SAR) was activated in non-transgenic poplars after S. populiperda incubation, and that induced systemic resistance (ISR) was activated more obviously in transgenic poplars after S. populiperda incubation. Hence, overexpression of PtDefensin may improve the resistance of poplar plants to pathogens.</div>
</front>
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<Abstract><AbstractText>Plant defensins have been implicated in the plant defense system, but their role in poplar immunity is still unclear. In the present study, we present evidence that PtDefensin, a putative plant defensin, participates in the defense of poplar plants against Septotis populiperda infection. After the construction of recombinant plasmid PET-32a-PtDefensin, PtDefensin protein was expressed in Escherichia coli strain BL21 (DE3) and purified through Ni-IDA resin affinity chromatography. The Trx-PtDefensin fusion protein displayed no cytotoxic activity against RAW264.7 cells but had cytotoxic activity against E. coli K12D31 cells. Analyses of PtDefensin transcript abundance showed that the expression levels of PtDefensin responded to abiotic and biotic stresses. Overexpression of PtDefensin in 'Nanlin 895' poplars (Populus × euramericana cv 'Nanlin895') increased resistance to Septotis populiperda, coupled with upregulation of MYC2 (basic helix-loop-helix (bHLH) transcription factor) related to jasmonic acid (JA) signal transduction pathways and downregulation of Jasmonate-zim domain (JAZ), an inhibitor in the JA signal transduction pathway. We speculate that systemic acquired resistance (SAR) was activated in non-transgenic poplars after S. populiperda incubation, and that induced systemic resistance (ISR) was activated more obviously in transgenic poplars after S. populiperda incubation. Hence, overexpression of PtDefensin may improve the resistance of poplar plants to pathogens.</AbstractText>
<CopyrightInformation>Copyright © 2019 The Author(s). Published by Elsevier B.V. All rights reserved.</CopyrightInformation>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wei</LastName>
<ForeName>Hui</ForeName>
<Initials>H</Initials>
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<Author ValidYN="Y"><LastName>Movahedi</LastName>
<ForeName>Ali</ForeName>
<Initials>A</Initials>
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<Author ValidYN="Y"><LastName>Xu</LastName>
<ForeName>Chen</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University. Nanjing, 210037, China; Jiangsu Provincial Key Construction Laboratory of Special Biomass Resource Utilization, Nanjing Xiaozhuang University, Nanjing, 211171, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Sun</LastName>
<ForeName>Weibo</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University. Nanjing, 210037, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Xiaoli</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University. Nanjing, 210037, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Dawei</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University. Nanjing, 210037, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Zhuge</LastName>
<ForeName>Qiang</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, College of Biology and the Environment, Nanjing Forestry University. Nanjing, 210037, China. Electronic address: qzhuge@njfu.edu.cn.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>12</Month>
<Day>17</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Ireland</Country>
<MedlineTA>Plant Sci</MedlineTA>
<NlmUniqueID>9882015</NlmUniqueID>
<ISSNLinking>0168-9452</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D023082">Defensins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D023082" MajorTopicYN="N">Defensins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">JAZ</Keyword>
<Keyword MajorTopicYN="N">MYC2</Keyword>
<Keyword MajorTopicYN="N">Populus trichocarpa</Keyword>
<Keyword MajorTopicYN="N">PtDefensin</Keyword>
<Keyword MajorTopicYN="N">SAR</Keyword>
<Keyword MajorTopicYN="N">Septotis populiperda</Keyword>
</KeywordList>
<CoiStatement>Declaration of Competing Interest The authors declare no conflicts of interest.</CoiStatement>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year>
<Month>09</Month>
<Day>14</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2019</Year>
<Month>12</Month>
<Day>04</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2019</Year>
<Month>12</Month>
<Day>13</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2020</Year>
<Month>2</Month>
<Day>2</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2020</Year>
<Month>2</Month>
<Day>2</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>9</Month>
<Day>1</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">32005384</ArticleId>
<ArticleId IdType="pii">S0168-9452(19)31552-3</ArticleId>
<ArticleId IdType="doi">10.1016/j.plantsci.2019.110379</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Wei, Hui" sort="Wei, Hui" uniqKey="Wei H" first="Hui" last="Wei">Hui Wei</name>
</noRegion>
<name sortKey="Li, Dawei" sort="Li, Dawei" uniqKey="Li D" first="Dawei" last="Li">Dawei Li</name>
<name sortKey="Movahedi, Ali" sort="Movahedi, Ali" uniqKey="Movahedi A" first="Ali" last="Movahedi">Ali Movahedi</name>
<name sortKey="Sun, Weibo" sort="Sun, Weibo" uniqKey="Sun W" first="Weibo" last="Sun">Weibo Sun</name>
<name sortKey="Wang, Xiaoli" sort="Wang, Xiaoli" uniqKey="Wang X" first="Xiaoli" last="Wang">Xiaoli Wang</name>
<name sortKey="Xu, Chen" sort="Xu, Chen" uniqKey="Xu C" first="Chen" last="Xu">Chen Xu</name>
<name sortKey="Zhuge, Qiang" sort="Zhuge, Qiang" uniqKey="Zhuge Q" first="Qiang" last="Zhuge">Qiang Zhuge</name>
</country>
</tree>
</affiliations>
</record>

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Overexpression of PtDefensin enhances resistance to Septotis populiperda in transgenic poplar.

Overexpression of PtDefensin enhances resistance to Septotis populiperda in transgenic poplar.

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